AffiGEL® Matrix GFR, Phenol Red-Free, LDEV-Free

AffiGEL® Matrix GFR, Phenol Red-Free, LDEV-Free

Equivalent: 356231 | Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free

Matrix type: Growth factor reduction

Application: Mainly to exclude the interference of growth factors on the experiment. Applied to related research on growth factors, signaling pathways, etc.

Product Information 

AffiGEL® Matrix GFR, Phenol Red-Free, LDEV-Free is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality

• Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Extensive PCR testing is performed to screen for a number of pathogens
• Tested and found negative for bacteria, fungi, and mycoplasma
• Protein concentrations determined by Lowry method
• Endotoxin units measured by Limulus Amoebocyte Lysate assay
• Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
• Biological activity is determined for each lot using a neurite outgrowth assay

Source

Engelbreth-Holm-Swarm Mouse Tumor

Preparation and Storage

Store at -20°C. Avoid multiple freeze-thaws. Do not store in frost-free freezer. KEEP FROZEN.

Application

• Cell Growth and Differentiation
• Metabolism/Toxicology Studies
• Invasion Assays
• in vitro and in vivo Angiogenesis Assays
• in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice