AffiGEL® Matrix High Concentration, Phenol Red-Free, LDEV-Free

Size: 10 mL
CORNING Matrigel Cat No. 354262

452.00 452.0 USD 452.00


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AffiGEL® Matrix High Concentration, Phenol Red-Free, LDEV-Free

Equivalent: 354262 | Corning® Matrigel® Matrix High Concentration (HC), Phenol-Red Free, LDEV-free

Matrix type: High concentration (≥18mg/ml)

Application: Experiments such as angiogenesis, gel embolization, and in vivo tumor formation (for angiogenesis,it is recommended that the final concentration of AffiGEL® basement membrane matrix should be ≥10mg/ml)

Product Information 

AffiGEL® Matrix High Concentration, Phenol Red-Free, LDEV-Free is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Preparation and Storage

Store at -20°C. Avoid multiple freeze-thaws.


Engelbreth-Holm-Swarm Mouse Tumor


  • Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
  • Extensive PCR testing is performed to screen for a number of pathogens
  • Tested and found negative for bacteria, fungi, and mycoplasma
  • Protein concentrations determined by Lowry method
  • Endotoxin units measured by Limulus Amoebocyte Lysate assay
  • Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
  • Biological activity is determined for each lot using a neurite outgrowth assay


  • Cell Growth and Differentiation
  • Metabolism/Toxicology Studies
  • Invasion Assays
  • in vitro and in vivo Angiogenesis Assays
  • in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice